| Catalogue Number |
CYT-216 |
| Synonyms |
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF. |
| Introduction |
The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003). |
| Description |
Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton.
IGF-I is purified by proprietary chromatographic techniques. |
| Source |
Escherichia Coli. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
The protein was lyophilized with no additives. |
| Solubility |
It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE. |
| Amino acid sequence |
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA. |
| Biological Activity |
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1.0ng/ml, corresponding to a specific activity of 1,000,000U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml. |
| Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard. |
| References |
1.
Title: Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis.
Publication: Journal of Endocrinology (2009) 202, 287–297.
Link:
http://joe.endocrinology-journals.org/content/202/2/287.full.pdf
|
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » IGF1 Gilthead Seabream |
| » IGF1 Human Des1-3 |
| » IGF1 Human, GST |
| » IGF1 Mouse |
| » IGF1 Rat |
| » Long R3 IGF1 Human |
|
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貨號CYT-211重組人白介素-4 IL-4 2µg ￥900
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Human HDL 高密度脂蛋白膽固醇 Specifications ：MF, AF, C (10mg/ml)；Source：Human blood ；Purity：98% 2mg 1200
DiI-HDL DiI 熒光標記高密度脂蛋白 pecifications ：MF, AF , C；Source：Human blood；Purity：98% ；300ug 2300
[I125] Human HDL 碘125標記人高密度脂蛋白pecifications ：MF, AF, (100-300cpm/ng)； Source：Human blood； 300uCi 2800
Human LDL人低密度脂蛋白 pecifications ：MF, AF, C (2-4mg/ml；Source：Human blood；Purity ：98% 2mg 1200
DiI-LDL DiI標記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2300
[I125] Human LDL 碘125標記人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng) , C；Source ：Human blood；Purity ：98% 100uCi 2600
DiO-LDL DiO標記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2700
Human Ac-LDL 人乙酰化低密度脂蛋白 pecifications ：MF, AF, (2mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ac-LDL DiI標記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 200ug 2800
Dio-Ac-LDL DiO標記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標記乙酰化人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng), C；Source ：Human blood；Purity ：98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (1.5-2.5mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ox-LDL DiI標記人氧化低密度脂蛋白 pecifications ：MF, AF, (200ug/Vial), C；Source ：Human blood 300ug 2300
[I125] Ox-LDL 碘125標記人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (100-300cpm/ng), C ；Source ：Human blood；Purity ：98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications ：Contains 0.01% Azide ；Source ：Human blood；Purity ：98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications ：MF, AF, C (1-2mg/ml) ；Source ：Human blood；Purity ：98% 2mg 1200
DiI-VLDL DiI標記的人極低密度脂蛋白
[I125] Human VLDL 碘125標記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
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