使用目的：本試劑盒用于測定進口/國產大鼠血清、血漿及相關液體樣本β內啡肽（β-EP）含量。 

本試劑僅供研究使用      標本：血清或血漿

注：本產品僅供科研使用！

The performance of kit:

1 sensitivity: minimum detection concentration is less than 1 standard. Liβ-EParity of dilution. Sample liβ-EPar regression and the expected concentration correlation coefficient R value is 0.990.

2: no specific reaction with other cytokiβ-EPs.

3 repeaβ-EPility: plate, plate between the coefficients of variation were less than 10%.

 試驗原理：

β-EP試劑盒是固相夾心法酶聯免疫吸附實驗（ELISA）.已知β-EP濃度的標準品、未知濃度的樣品加入微孔酶標板內進行檢測。先將β-EP和生物素標記的抗體同時溫育。洗滌后，加入親和素標記過的HRP。再經過溫育和洗滌，去除未結合的酶結合物，然后加入底物A、B，和酶結合物同時作用。產生顏色。顏色的深淺和樣品中β-EP的濃度呈比例關系。

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