1.Human CD54 / ICAM-1 ELISA KIT Intended use
The Diaclone Human sICAM-1/CD54 ELISA is to be used for the in-vitro quantitative determination of soluble Inter Cellular Adhesion Molecule -1 (sICAM-1) in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human sICAM-1/CD54. This kit has been configured for research use only.

2. Human CD54 / ICAM-1 ELISA KIT  Principle of the method
The CD54 Kit is a solid phase sandwich Enzyme linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD54 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD54 concentrations and unknowns are pipetted into these wells.

During the first incubation, the CD54 antigen and a biotinylated monoclonal antibody specific for CD54 are simultaneously incubated.

After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of  this coloured product is directly proportional to the concentrationof CD54 present in the samples.

公司網址：www.craymeibio.com