| Catalogue Number |
CYT-216 |
| Synonyms |
Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF. |
| Introduction |
The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003). |
| Description |
Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7655 Dalton.
IGF-I is purified by proprietary chromatographic techniques. |
| Source |
Escherichia Coli. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
The protein was lyophilized with no additives. |
| Solubility |
It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 95.0% as determined by
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE. |
| Amino acid sequence |
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA. |
| Biological Activity |
The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 1.0ng/ml, corresponding to a specific activity of 1,000,000U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20 ng/ml. |
| Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard. |
| References |
1.
Title: Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis.
Publication: Journal of Endocrinology (2009) 202, 287–297.
Link:
http://joe.endocrinology-journals.org/content/202/2/287.full.pdf
|
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » IGF1 Gilthead Seabream |
| » IGF1 Human Des1-3 |
| » IGF1 Human, GST |
| » IGF1 Mouse |
| » IGF1 Rat |
| » Long R3 IGF1 Human |
|
2011年底 生化試劑-大促銷
另有大量贈品贈送 歡迎來電咨詢！
貨號A1350,1000 Citric acid monohydrate Buffer grade 無水檸檬酸 1kg ￥365
貨號A0427,0010 Safranine O (C.I. 50240) for Microscopy 番紅O/沙黃O 10g ￥461
貨號A0664,0001 Xanthosine dihydrate BioChemica 黃嘌呤核苷 1g ￥360
貨號A0670,0005 Thymine 胸腺嘧啶 5g ￥320
貨號A0683,0010 Neutral red (C.I.50040) 中性紅 10g ￥455
貨號A0691,0025 Crystal violet (C.I. 42555) 結晶紫 25g ￥376
貨號A0785,0500 Choline chloride BioChemica 氯化膽堿 500g ￥731
貨號A0839,0010 Ampicillin sodium salt BioChemica 氨芐青霉素鈉鹽 10g ￥426
貨號A0878,0005 EGTA Molecular biology grade 乙二醇雙(2-氨基乙基)四乙酸 5g ￥394
貨號A0890,0001 Polymyxin B sulfate BioChemica 多粘菌素B硫酸鹽 1g ￥695
貨號A0939,0010 Adenine BioChemica 腺嘌呤 10g ￥365
貨號A1024,0100 Bicine Buffer grade N-二甘氨酸/N-二羥乙基甘氨酸 100g ￥733
貨號A1051,0001 Bromocresol green 溴甲酚綠 1g ￥344
貨號A1060,0025 ACES Buffer grade ACES緩沖液 25g ￥556
貨號A1065,0025 CHES Buffer grade CHES緩沖液 25g ￥418
貨號A1070,0050 HEPES sodium salt Buffer grade HEPES鈉鹽 50g ￥628
貨號A1078,0025 MOPSO Buffer grade MOPSO緩沖液 25g ￥450
貨號A1085,0100 Tricine BioChemica 三(羥甲基)甲基甘氨酸 100g ￥733
貨號A1104,0250 EDTA disodium salt dihydrate 乙二胺四乙酸二鈉/EDTA二鈉 250g ￥389
貨號A1148,0025 TEMED 四甲基乙二胺 25ml ￥320
貨號A1308,0010 S-MEM_ modified for spinner culture S-MEM 1L ￥400
貨號A1344,0100 L-Phenylalanine pure Ph. Eur., USP L-苯丙氨酸 100g ￥635
貨號A1345,0100 L-Arginine base pure Ph. Eur., USP L-精氨酸 100g ￥398
貨號A1350,1000 Citric acid monohydrate Buffer grade 無水檸檬酸 1kg ￥365
貨號A1379,0500 Tris Buffer grade Tris緩沖液 500g ￥628
貨號A1380,0010 Benzamidine hydrochloride BioChemica 鹽酸芐脒/鹽酸苯甲脒 10g ￥559
貨號A1381,0500 Brij® 35 BioChemica Brij® 35 500g ￥702
貨號A1390,0500 Tween® 80 BioChemica 吐溫80 500ml ￥493
貨號A1401,0025 Fast Green FCF (C.I. 42053) 快綠 25g ￥1,010
貨號A1406,0005 Pyronin Y (C.I.45005) 派洛寧Y 5g ￥1,129
貨號A1408,0010 Xylene cyanol FF (C.I. 42135) BioChemica 二甲苯胺 10g ￥876
貨號A1441,0250 Trypsin inhibitor from soybean >10000 BAEE 胰蛋白酶抑制劑 250mg ￥1,074
貨號A1491,0001 Carbenicillin disodium salt BioChemica 羥芐青霉素二鈉 1g ￥514
貨號A1492,0001 Gentamycin sulfate BioChemica 慶大霉素硫酸鹽 1g ￥320
貨號A1493,0005 Kanamycin sulfate BioChemica 硫酸卡那霉素 5g ￥593
貨號A1553,0250 Tryptone BioChemica 胰蛋白胨 250g ￥797
貨號A1561,0001 Bilirubin pure 膽紅素 1g ￥1,262
貨號A1795,0025 Fuchsin basic (C.I. 42510) Microscopy grade 品紅/洋紅 堿性 25g ￥389
貨號A1828,0001 Trypsin inhibitor from soybean >7000 BAEE 胰蛋白酶抑制劑 1g ￥1,334
貨號A1828,0250 Trypsin inhibitor from soybean >7000 BAEE 胰蛋白酶抑制劑 250mg ￥516
貨號A1847,0025 Dextran 70000 BioChemica 葡聚糖 7000 25g ￥398
貨號A1907,0050 Amphotericin B BioChemica 兩性霉素B 50mg ￥439
貨號A1907,0250 Amphotericin B BioChemica 兩性霉素B 250mg ￥1,448
貨號A2084,0005 L-Glutathione reduced BioChemica L-谷胱甘肽 還原型 5g ￥444
貨號A2115,0050 Agarose high EEO 瓊脂糖 高EEO 50g ￥1,403
貨號A2116,0050 Agarose medium EEO 瓊脂糖 中EEO 50g ￥1,403
貨號A2144,0005 Chymostatin 抑凝乳蛋白酶素/抑糜蛋白酶素 5mg ￥1,309
貨號A2147,0025 β-Cyclodextrin BioChemica β-環糊精 25g ￥595
貨號A2163,0010 Flavinadenindinukleotid - Dinatriumsalz - Dihydrat BioChemica 黃素腺嘌呤二核苷酸 100mg ￥641
貨號A2175,0001 Hygromycin B - Solution 潮霉素B溶液 1ml ￥1,403
貨號A2202,0025 Genistein BioChemica 染料木黃酮/染料木堿/染料木素 25mg ￥1,186
貨號A2204,0500 Polyethylene glycol 8000 BioChemica (PEG8000) PEG8000 500g ￥320
貨號A2221,0500 Salicylic acid pure Ph. Eur., USP 水楊酸 500g ￥365
貨號A2228,0025 Tetracycline hydrochloride BioChemica 四環素鹽酸鹽 25g ￥320
貨號A2243,0001 L-Glutathione oxidized BioChemica L-谷胱甘肽 氧化型 1g ￥439
貨號A2249,0025 Dextran 40000 BioChemica 葡聚糖 4000 25g ￥398
貨號A2298,0025 Brilliant green (C.I. 42040) 亮綠/艷綠 25g ￥320
貨號A2403,0500 Sodium citrate dihydrate p. A. 二水檸檬酸鈉 500g ￥410
貨號A2760,0100 RNase A RNA酶A 100mg ￥556
貨號A2947,0100 MOPS Molecular biology grade MOPS 100g ￥882
貨號A2948,0005 DTT Molecular biology grade 二硫蘇糖醇/DTT 5g ￥826
貨號A3009,0250 Dimethyl sulfoxide pure Ph. Eur. 二甲基亞砜 250ml ￥320
貨號A3427,0500 Tris-Tricine-SDS buffer (5X) BioChemica Tris-Tricine-SDS buffer (5X) 500ml ￥532
貨號A3615,0001 Peroxidase from horseradish EIA and Immunology Grade I 過氧化物酶 1KU ￥1,050
貨號A3711,0001 Lysozyme BioChemica 溶菌酶 1g ￥354
貨號A3762,0010 Agarose Low Melt Large DNA grade 瓊脂糖 低熔點（大DNA片段） 10g ￥1,513
貨號A3787,0500 α-Chymotrypsin from bovine pancreas Grade II胰凝乳蛋白酶/糜蛋白酶 II級 500mg ￥510
貨號A3930,0025 Rhodamin B (C.I. 45170) 羅丹明B 25g ￥352
貨號A4011,0005 CHAPS Molecular biology grade CHAPS 5g ￥1,728
貨號A4065,0005 DL-α-Lipoic acid BioChemica DL-α-硫辛酸 5g ￥778
貨號A4090,0025 Lipase from porcine pancreas 脂肪酶 （豬） 25g ￥508
貨號A4148,0025 Trypsin 1:250 from porcine pancreas 豬胰蛋白酶（1:250） 25g ￥1,453
貨號A4259,0250 SDS pure Ph. Eur 十二烷基磺酸鈉 250g ￥386
貨號A4289,0025 Pepsin 胃蛋白酶 25g ￥386
貨號A4338,0010 β-Mercaptoethanol BioChemica β-巰基乙醇 100ml ￥358
貨號A4392,0001 Proteinase K - Solution 蛋白酶K溶液 1ml ￥463
貨號A4412,0100 DNA Marker Phage Lambda - BstE II (lyophilised) DNA Marker （入噬菌體 酶切） 100μg ￥953
貨號A4522,0100 tri-Sodium citrate dihydrate Molecular biology grade 二水檸檬酸鈉 100g ￥334
貨號A4532,0250 Trypsinogen 胰蛋白酶原 250mg ￥834
貨號A4619,00005 Antifade Antifade 0,5ml ￥318
貨號A4669,0001 Glucose Oxidase from Aspergillus niger, low catalase 葡萄糖氧化酶 1ml ￥709
貨號A4725,0100 Tris : Tris · HCl - Mixture (pH 8.8) Tris :HCl (pH 8.8) 100g ￥1,334
貨號A4730,0050 MES monohydrate Molecular Biology grade MES(一水) 50g ￥545
貨號A4942,0005 Taq DNA Polymerase Reaction buffer (10X) complete Taq DNA聚合酶 反應緩沖液 5ml ￥320
貨號A4978,0100 X-Gal Molecular Biology grade X-Gal 100mg ￥424
貨號A5069,0010 Coenzyme Q10 BioChemica 輔酶Q /泛醌 10mg ￥410
貨號A5194,0005 DNA Marker Phage Lambda - Sty I DNA Marker （入噬菌體 酶切） 50μg ￥457
貨號A5196,0005 DNA pUC19 Plasmid pUC19質粒DNA 0,05mg ￥1,585
貨號A5197,0005 DNA Phage T7 T7噬菌體DNA 0.05mg ￥2,398
貨號A5220,0005 DNA Marker Phage Lambda - BstE II DNA Marker （入噬菌體 酶切） 50μg ￥434
貨號A5223,0005 DNA Marker Phage Lambda - Hind III DNA Marker （入噬菌體 酶切） 50μg ￥347
貨號A5238,0500 Protein Marker I (14-116) 蛋白質marker I 14-116kD 500μl ￥738
貨號A5434,0100 Taq DNA Polymerase DNA-free Taq DNA聚合酶 100U/ml ￥850
貨號A5474,0025 Rhodamine 6G (C.I. 45160) 羅丹明6G 25g ￥445
貨號A5589,0100 DNA Marker Phage Lambda Hind III (lyophilised) DNA Marker （入噬菌體 酶切） 100μg ￥947
貨號A6352,0025 Ampicillin sodium salt pure Ph. Eur. 氨芐青霉素鈉鹽 25g ￥826
貨號A6435,0050 Chloramphenicol pure Ph. Eur. 氯霉素 50g ￥354
貨號A6611,0010 Albumin_ human 白蛋白 （人） 10mg ￥521
貨號A6611,0100 Albumin, human 白蛋白 （人） 100mg ￥461
貨號A6775,0005 Azure B (C.I. 52010) 天青B/天藍B /三甲硫紫 5g ￥676
貨號A6872,0250 D-Glucosamine hydrochloride BioChemica D-葡萄糖胺 250g ￥1,207
貨號A6932,0500 Bradford - Solution for Protein determination Bradford蛋白定量溶液 500ml ￥1,223
貨號A6980,0025 Pepsinogen II, human 胃蛋白酶原II，人源 25μg ￥1,133
貨號A7002,0025 Neomycin sulfate pure Ph. Eur. 新霉素 25g ￥432
貨號A7003,0100 Streptomycin sulfate pure Ph. Eur. 硫酸鏈霉素 100g ￥950
貨號A7016,500 TBS (Tris-buffered saline, 10X) TBS 500ml ￥1,069
貨號A7086,0001 Lithium 3,5-diiodosalicylate 3,5-二碘水楊酸鋰 1g ￥628
貨號A7282,0250 Betaine hydrochloride pure 甜菜堿 250g ￥553
貨號A7686,0005 5-Fluorouracil (BioChemica) 5-氟尿嘧啶 5g ￥613
貨號A7686,0005 5-Fluorouracil (BioChemica) 5-氟尿嘧啶 5g ￥613
貨號CYT-201 重組人紅細胞生成素-a EPO-a 5µg ￥900
貨號CYT-209 重組人白介素-2 Human IL-2 10µg ￥900
貨號CYT-211重組人白介素-4 IL-4 2µg ￥900
貨號CYT-213 重組人白介素-6 IL-6 5µg ￥900
貨號CYT-216 重組人胰島素樣生長因子-1 Human IGF-1 20µg ￥900
貨號CYT-222A 重組小鼠粒細胞巨噬細胞集落刺激因子 mGMCSF 2μg ￥900
貨號CYT-223 重組人腫瘤壞死因子-a hTNF-a 10µg ￥900
貨號CYT-227 重組大鼠瘦素 rLeptin 20µg ￥900
貨號CYT-241A 重組人血管內皮生長因子 VEGF 2μg ￥900
貨號CYT-280 重組人脂聯素 Acrp30 5µg ￥900
貨號CYT-289 重組大鼠胰島素樣生長因子-1 rIGF-1 10μg ￥900
貨號CYT-318 重組人內脂素 Visfatin Hu 25µg ￥1,900
貨號CYT-318 重組人內脂素 Visfatin Hu 5µg ￥900
貨號CYT-326 重組小鼠表皮生長因子 mEGF 100µg ￥900
貨號CYT-336 重組小鼠血管內皮生長因子 mVEGF 2µg ￥900
貨號CYT-382A 重組大鼠白介素-2 rIL-2 5µg ￥900
貨號CYT-388 重組大鼠白介素-6 rIL-6 2µg ￥900
貨號CYT-394 重組大鼠白介素-1β rIL 1 beta 2µg ￥900
貨號CYT-405 重組豬腫瘤壞死因子-a rpTNF-a 5µg ￥900
貨號CYT-446A 重組人白介素-13 IL 13 2µg ￥900
貨號CYT-569 重組人活化素-A Activin-A 2µg ￥900
貨號CYT-569 重組人活化素-A Activin-A 2µg ￥900
貨號CYT-580 重組人色素上皮衍生因子 PEDF 20µg ￥1,900
貨號ENZ-308 Taq DNA聚合酶（4支一套） TaqDNA 1,000U ￥900
貨號PRO-445 U1snRNP-68 U1snRNP-68 20µg ￥1,900
Human HDL 高密度脂蛋白膽固醇 Specifications ：MF, AF, C (10mg/ml)；Source：Human blood ；Purity：98% 2mg 1200
DiI-HDL DiI 熒光標記高密度脂蛋白 pecifications ：MF, AF , C；Source：Human blood；Purity：98% ；300ug 2300
[I125] Human HDL 碘125標記人高密度脂蛋白pecifications ：MF, AF, (100-300cpm/ng)； Source：Human blood； 300uCi 2800
Human LDL人低密度脂蛋白 pecifications ：MF, AF, C (2-4mg/ml；Source：Human blood；Purity ：98% 2mg 1200
DiI-LDL DiI標記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2300
[I125] Human LDL 碘125標記人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng) , C；Source ：Human blood；Purity ：98% 100uCi 2600
DiO-LDL DiO標記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2700
Human Ac-LDL 人乙酰化低密度脂蛋白 pecifications ：MF, AF, (2mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ac-LDL DiI標記乙?；说兔芏戎鞍?pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 200ug 2800
Dio-Ac-LDL DiO標記乙?；说兔芏戎鞍?pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標記乙?；说兔芏戎鞍?pecifications ：MF, AF, (100-300cpm/ng), C；Source ：Human blood；Purity ：98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (1.5-2.5mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ox-LDL DiI標記人氧化低密度脂蛋白 pecifications ：MF, AF, (200ug/Vial), C；Source ：Human blood 300ug 2300
[I125] Ox-LDL 碘125標記人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (100-300cpm/ng), C ；Source ：Human blood；Purity ：98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications ：Contains 0.01% Azide ；Source ：Human blood；Purity ：98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications ：MF, AF, C (1-2mg/ml) ；Source ：Human blood；Purity ：98% 2mg 1200
DiI-VLDL DiI標記的人極低密度脂蛋白
[I125] Human VLDL 碘125標記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
本頁產品地址：http://www.blueally.cn/sell/show-183773.html
免責聲明：以上所展示的[貨號CYT-216 重組人胰島素樣生長因子-1]信息由會員[上海經科化學科技有限公司]自行提供，內容的真實性、準確性和合法性由發布會員負責。
