Grade:BIOTECHNOLOGY GRADE

CAS Number: 3483-12-3

Synonym(s): dithiothreitol, dtt, Cleland's reagent

Molecular Weight: 154.25

Molecular Formula: C₄H₁₀O₂S₂

Storage Condition: FROZEN

Hazard Statements: H302 / H315 / H319 / H335

Precautionary Statements: P280 / P301+P312 / P302+P352 / P304+P340 / P305+P351+P338 / P403+P233

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Abs.@260nm (0.1M, Water) 。。。。。。。。。。。。。。。。 REPORT

Abs.@280nm (0.1M, Water) 。。。。。。。。。。。。。。。。REPORT

Abs.@283nm (0.02M, Water)。。。。。。。。。。。。。。。。 0.05

DNase (P/F) 。。。。。。。。。。。。。。。。。。。。。。。。。NONE

IR (P/F)。。。。。。。。。。。。。。。。。。。。。。。。。。。PASS

Loss on Drying (%)。。。。。。。。。。。。。。。。。。。。。0.5

Melting Point (°C) 。。。。。。。。。。。。。。。。。。。。。39 - 43

Oxidized DTT (%)。。。。。。。。。。。。。。。。。。。。。。0.5

Protease (P/F) 。。。。。。。。。。。。。。。。。。。。。。。NONE

Purity (-SH Content) (%)。。。。。。。。。。。。。。。。。。99.4

Reassay Date 。。。。。。。。。。。。。。。。。。。。。。。。REPORT

RNase (P/F)。。。。。。。。。。。。。。。。。。。。。。。。。NONE

Solubility (5%, Water) (P/F) 。。。。。。。。。。。。。。。PASS